E-mail Alert
RSS
Design of non-invasive skin cholesterol detection system based on absorption spectroscopy
-
摘要
利用STM32微处理器，设计了一种基于吸收光谱技术的皮肤胆固醇无创检测系统，通过微型光谱仪获得有色产物的吸收光谱信息，间接得到人体皮肤胆固醇相对含量。本系统设计了一款高精度可调LED恒流源，LED光强的波动范围控制在±1%以内。还设计了一种结构简单、试剂用量少、不需要确切的检测试剂体积的液体限位装置，实现被测液体浓度的准确测量。通过检测梯度浓度CuSO4溶液，验证了系统应用于不同浓度溶液定量检测的准确性。采用本系统对动脉粥样硬化性疾病患者和对照人群的皮肤胆固醇进行检测，检测结果在统计学上有显著性差异，初步验证系统可用于人体皮肤胆固醇的检测。
Abstract
Using STM32 microprocessor, a non-invasive skin cholesterol detection system based on absorption spectroscopy was designed. The relative cholesterol content of human skin was indirectly obtained by absorption spectrum information of colored products which was detected by micro-spectrometer. The system was designed with a high-precision adjustable LED constant current source, and the fluctuation range of LED light intensity is controlled within ±1%. A liquid limit device with a simple structure, a small amount of reagents, and no need for an exact detection reagent volume was also designed to achieve accurate measurement of the measured liquid concentration. By detecting the concentration of CuSO4 solution, the accuracy of the system for quantitative detection of different concentrations of solution was verified. Using this system to detect the skin cholesterol of patients with atherosclerotic disease and control population, the test results have statistically significant differences, which preliminarily verifies that system can be used for human skin cholesterol detection.
-
Overview
Overview: Studies have shown that the accumulation of cholesterol in human skin correlates with the risk of developing atherosclerotic disease. Skin cholesterol has a better correlation with the risk of atherosclerotic disease compared to traditional blood cholesterol. A simple, noninvasive procedure "Three Drops" for estimation of skin cholesterol was proposed as an alternative screening method. The test, which uses different concentrations of a digitonin–copolymer– horseradish peroxidase (HRP) conjugate and visual scoring, is capable of evaluating different skin cholesterol levels. According to the procedure, researchers have proposed a skin cholesterol detection method based on diffuse reflectance spectroscopy technology. This method directly detect the diffuse reflectance spectrum information of the reagent after the reaction on the skin surface. Because this method is directly measured on the palm surface, many interference factors are introduced. Using STM32 microprocessor, a non-invasive skin cholesterol detection system based on absorption spectroscopy was designed. The relative cholesterol content of human skin was indirectly obtained by absorption spectrum information of colored products which was detected by micro-spectrometer. The system was designed with a high-precision adjustable LED constant current source, and the fluctuation range of LED light intensity is controlled within ±1%. A liquid limit device with a simple structure, a small amount of reagents, and no need for an exact detection reagent volume was also designed to achieve accurate measurement of the measured liquid concentration. By detecting the concentration of CuSO4 solution, the accuracy of the system for quantitative detection of different concentrations of solution was verified. Using this system to detect the skin cholesterol of patients with atherosclerotic disease and control population, the test results have statistically significant differences, which preliminarily verifies the system can be used for human skin cholesterol detection.
-
-
参考文献
[1] Bouissou H, Pieraggi M T, Julian M, et al. Identifying arteriosclerosis and aortic atheromatosis by skin biopsy[J]. Atherosclerosis, 1974, 19(3): 449–458. doi: 10.1016/S0021-9150(74)80009-2
[2] Zawydiwski R, Sprecher D L, Evelegh M J, et al. A novel test for the measurement of skin cholesterol[J]. Clinical Chemistry, 2001, 47(7): 1302–1304.
[3] Tashakkor A Y, Mancini G B J. The relationship between skin cholesterol testing and parameters of cardiovascular risk: a systematic review[J]. Canadian Journal of Cardiology, 2013, 29(11): 1477–1487. doi: 10.1016/j.cjca.2013.04.007
[4] Sprecher D L, Goodman S G, Kannampuzha P, et al. Skin tissue cholesterol (SkinTc) is related to angiographically-defined cardiovascular disease[J]. Atherosclerosis, 2003, 171(2): 255–258. doi: 10.1016/S0021-9150(03)00290-9
[5] Tzou W S, Mays M E, Korcarz C E, et al. Skin cholesterol content identifies increased carotid intima-media thickness in asymptomatic adults[J]. American Heart Journal, 2005, 150(6): 1135–1139. doi: 10.1016/j.ahj.2005.01.009
[6] Lopukhin Y L. Skin and Atherosclerosis (a three-drop test)[M]. Berkshire, UK: Harwood Academic Publishers, 1992: 1–124.
[7] 候华毅, 董美丽, 王贻坤, 等.漫反射光谱技术快速无创检测皮肤胆固醇[J].光谱学与光谱分析, 2016, 36(10): 3215–3221. http://www.opticsjournal.net/Articles/abstract?aid=OJ161230000121RoUqXt
Hou H Y, Dong M L, Wang Y K, et al. Rapid and noninvasive detection of skin cholesterol with diffuse reflectance spectroscopy technology[J]. Spectroscopy and Spectral Analysis, 2016, 36(10): 3215–3221. http://www.opticsjournal.net/Articles/abstract?aid=OJ161230000121RoUqXt
[8] 李晨曦, 孙哲, 韩蕾, 等.基于漫反射光谱的组织光学参数测量系统与方法研究[J].光谱学与光谱分析, 2016, 36(5): 1532–1536. http://industry.wanfangdata.com.cn/yj/Detail/Periodical?id=Periodical_gpxygpfx201605054
Li C X, Sun Z, Han L, et al. Study on the determination system of tissue optical properties based on diffuse reflectance spectrum[J]. Spectroscopy and Spectral Analysis, 2016, 36(5): 1532–1536. http://industry.wanfangdata.com.cn/yj/Detail/Periodical?id=Periodical_gpxygpfx201605054
[9] 候华毅, 方朝晖, 张元志, 等.皮肤胆固醇无创光谱检测模拟和在体实验研究[J].中国激光, 2016, 43(9): 0907001. http://www.doc88.com/p-2428959458927.html
Hou H Y, Fang Z H, Zhang Y Z, et al. Simulation and in vivo experimental study on noninvasive spectral detection of skin cholesterol[J]. Chinese Journal of Lasers, 2016, 43(9): 0907001. http://www.doc88.com/p-2428959458927.html
[10] Matsumoto H S H, Ota I, Nagaoka T. Detection of skin cholesterol by a molecularly imprinted electrode[J]. Journal of Flow Injection Analysis, 2008, 25(1): 81–84. https://ci.nii.ac.jp/naid/40016198356
[11] Mancini G B J, Chan S, Frohlich J, et al. Association of skin cholesterol content, measured by a noninvasive method, with markers of inflammation and Framingham risk prediction[J]. The American Journal of Cardiology, 2002, 89(11): 1313–1316. doi: 10.1016/S0002-9149(02)02336-6
[12] 周志敏, 周纪海, 纪爱华. LED驱动电路设计与应用[M].北京:电子工业出版社, 2008.
[13] 来清民.高亮度LED照明及驱动电路设计[M].北京:北京航空航天大学出版社, 2012.
[14] Sprecher D L, Pearce G L. Elevated skin tissue cholesterol levels and myocardial infarction[J]. Atherosclerosis, 2005, 181(2): 371–373. doi: 10.1016/j.atherosclerosis.2005.01.022
[15] Sprecher D L, Pearce G L. Skin cholesterol adds to Framingham risk assessment[J]. American Heart Journal, 2006, 152(4): 694–696. doi: 10.1016/j.ahj.2006.04.027
-
访问统计
下载:
点击扫一扫
图(13)
计量
- 文章访问数:
- PDF下载数:
- 施引文献: 0
